| Registro completo |
| Provedor de dados: |
ArchiMer
|
| País: |
France
|
| Título: |
Archaeal communities associated with shallow to deep subseafloor sediments of the New Caledonia Basin
|
| Autores: |
Roussel, Erwan
Sauvadet, Anne-laure
Chaduteau, Carine
Fouquet, Yves
Charlou, Jean-luc
Prieur, Daniel
Cambon, Marie-anne
|
| Data: |
2009-09
|
| Ano: |
2009
|
| Palavras-chave: |
Marine subsurface sediments
Sea floor biosphere
Gradient gel electrophoresis
Microbial communities
Molecular diversity
Anaerobic oxidation
Sequence alignment
Phylogenetic trees
Extracellular DNA
Methane hydrate
|
| Resumo: |
P>The distribution of the archaeal communities in deep subseafloor sediments [0-36 m below the seafloor (mbsf)] from the New Caledonia and Fairway Basins was investigated using DNA- and RNA-derived 16S rRNA clone libraries, functional genes and denaturing gradient gel electrophoresis (DGGE). A new method, Co-Migration DGGE (CM-DGGE), was developed to access selectively the active archaeal diversity. Prokaryotic cell abundances at the open-ocean sites were on average similar to 3.5 times lower than at a site under terrestrial influence. The sediment surface archaeal community (0-1.5 mbsf) was characterized by active Marine Group 1 (MG-1) Archaea that co-occurred with ammonia monooxygenase gene (amoA) sequences affiliated to a group of uncultured sedimentary Crenarchaeota. However, the anoxic subsurface methane-poor sediments (below 1.5 mbsf) were dominated by less active archaeal communities, such as the Thermoplasmatales, Marine Benthic Group D and other lineages probably involved in the methane cycle (Methanosarcinales, ANME-2 and DSAG/MBG-B). Moreover, the archaeal diversity of some sediment layers was restricted to only one lineage (Uncultured Euryarchaeota, DHVE6, MBG-B, MG-1 and SAGMEG). Sequences forming two clusters within the Thermococcales order were also present in these cold subseafloor sediments, suggesting that these uncultured putative thermophilic archaeal communities might have originated from a different environment. This study shows a transition between surface and subsurface sediment archaeal communities.
|
| Tipo: |
Text
|
| Idioma: |
Inglês
|
| Identificador: |
http://archimer.ifremer.fr/doc/2009/publication-6801.pdf
DOI:10.1111/j.1462-2920.2009.01976.x
|
| Editor: |
Wiley / Blackwell
|
| Relação: |
http://archimer.ifremer.fr/doc/00000/6801/
|
| Formato: |
application/pdf
|
| Fonte: |
Environmental Microbiology (1462-2912) (Wiley / Blackwell), 2009-09 , Vol. 11 , N. 9 , P. 2446-2462
|
| Direitos: |
2009 Society for Applied Microbiology and Wiley - Blackwell Publishing Ltd.
|
|
